A practical protocol for secreted glycoprotein production in Pichia, drawn from 2,400+ published sources
2213
Papers
27
Authors
0
Sent
0
Replies
<2016
Filtered out
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
Monolith affinity chromatography for the rapid quantification of a single‐chain variable fragment immunotoxin
Small P particles formed by the Taiwan-native norovirus P domain overexpressed in Komagataella pastoris
Buried Kex2 Sites in Glargine Precursor Aggregates Prevent Its Intracellular Processing in Pichia pastoris Muts Strains
Novel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris
Controlling methanol feeding for recombinant protein production by Pichia pastoris under oxidation stress in fed-batch f
Enhancing the expression of recombinant small laccase in Pichia pastoris by a double promoter system and application in
Improvement of the recombinant phytase expression by intermittent feeding of glucose during the induction phase of methy
Sorbitol/methanol mixed induction reduces process impurities and improves centrifugal dewatering in Pichia pastoris cult
Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess
Combining expression and process engineering for high-quality production of human sialyltransferase in Pichia pastoris.
Substitution of the 5'-untranslated region in the alcohol oxidase 1 promoter of Komagataella phaffii alleviated the
High-level expression of Humicola insolens cutinase in Pichia pastoris without carbon starvation and its use in cotton f
Production in stirred-tank bioreactor of recombinant bovine chymosin B by a high-level expression transformant clone of
The molecular modification, expression, and the antibacterial effects studies of human lysozyme.
[High-level production of glucose oxidase by recombinant Pichia pastoris using a combined strategy].
Efficient Heterologous Production of Rhizopus oryzae Lipase via Optimization of Multiple Expression-Related Helper Prote
Bioprocess and downstream optimization of recombinant human growth hormone in Pichia pastoris.
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Biocalorimetric monitoring of glycoengineered P. pastoris cultivation for the production of recombinant huIFNα2b: A quan
Downregulation by organic nitrogen of AOX1 promoter used for controlled expression of foreign genes in the yeast Pichia
Kinetic Modeling of Pichia pastoris Bioreactor Systems Under Different Operating Conditions: Batch, Fed-Batch, and Chemo
Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by K
Impact of glycerol feeding profiles on STEAP1 biosynthesis by Komagataella pastoris using a methanol-inducible promoter.
Enhancing xylanase expression of Komagataella phaffii induced by formate through Mit1 co-expression.
A comparative study of glycerol and sorbitol as co-substrates in methanol-induced cultures of Pichia pastoris: temperatu
Influence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with li
Medium optimization for enhanced production of recombinant lignin peroxidase in Pichia pastoris.
Enhanced truncated-t-PA (CT-b) expression in high-cell-density fed-batch cultures of Pichia pastoris through optimizatio
Towards optimal substrate feeding for heterologous protein production in <i>Pichia pastoris (Komagataella spp)</i> fed‐b
Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Base
Applied Microbiology 2016- Pichia process optimization by methanol/sorbitol co-feeding – Patrick Fickers - Universi
Bioprocess efficiency as a roadmap to establish Pichia pastoris as a reliable cell factory
Implementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by
Methanol Independent Expression by <em>Pichia Pastoris</em> Employing De-repression Technologies
Optimized expression of capsid protein from red-grouper necrosis virus displayed on Pichia pastoris in shake flasks
Isolation of the Astacin-like metalloprotease coding gene (astl) and assessment of its insecticidal activity towards Spo
Expression and Purification of Human Lysosomal β-galactosidase from Pichia Pastoris
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
Microscale Perfusion‐Based Cultivation for <i>Pichia pastoris</i> Clone Screening Enables Accelerated and Optimized Reco
Improving phytase production in <i>Pichia pastoris</i> fermentations through de‐repression and methanol induction optimi
Heterologous expression of Neurospora crassa cbh1 gene in Pichia pastoris resulted in production of a neutral cellobiohy
Investigating the effect of carbon source on rabies virus glycoprotein production in<i>Pichia pastoris</i>by a transcrip
Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris
A highly versatile fungal glucosyltransferase for specific production of quercetin-7-O-β-D-glucoside and quercetin-3-O-β
Aspergillus oryzae S2 AmyA amylase expression in Pichia pastoris: production, purification and novel properties.
Optimized expression of prolyl aminopeptidase in Pichia pastoris and its characteristics after glycosylation.
Expression and Control of Codon-Optimized Granulocyte Colony-Stimulating Factor in Pichia pastoris.
Enhanced protein production by sorbitol co-feeding with methanol in recombinant Pichia pastoris strains
Expression of Bacillus licheniformis α-amylase in Pichia pastoris without antibiotics-resistant gene and effects of glyc
High-level expression and characterization of Aspergillus niger ATCC 1015 xylanase B in Komagataella phaffii
Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-β-endoglucanase.
Introduction of a glycosylation site in the constant region decreases the aggregation of adalimumab Fab.
Characterization and application in recombinant N-GlcNAc-protein production of a novel endo-β-N-acetylglucosaminidase fr
Production, purification, and characterization of recombinant rabies virus glycoprotein expressed in PichiaPink™ yeast.
High-level expression and characterization of a novel serine protease in Pichia pastoris by multi-copy integration.
Expression of a novel α-glucosidase from Aspergillus neoniger in Pichia pastoris and its efficient recovery for synthesi
Constitutive expression of Camelus bactrianus prochymosin B in Pichia pastoris.
Heterologous expression and biochemical characterization of a novel thermostable Sclerotinia sclerotiorum GH45 endogluca
Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interle
High-level expression of a novel α-amylase from Thermomyces dupontii in Pichia pastoris and its application in maltose s
Heterologous expression and characterization of an antifungal chitinase (Chit46) from Trichoderma harzianum GIM 3.442 an
Effects of N-linked glycosylation on the enzymatic properties of GH12 bifunctional enzymes from Aspergillus terreus expr
Deglycosylation significantly affects the activity, stability and appropriate folding of recombinant Aspergillus niger α
Fructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expr
An effective and simplified DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris.
Co-expression of Pseudomonas alcaligenes lipase and its specific foldase in Pichia pastoris by a dual expression cassett
Stabilizing Leaf and Branch Compost Cutinase (LCC) with Glycosylation: Mechanism and Effect on PET Hydrolysis.
Improving the Catalytic Efficiency of Aspergillus fumigatus Glucoamylase toward Raw Starch by Engineering Its N-Glycosyl
Enhancing the Thermostability and Catalytic Activity of the Lipase from Rhizopus oryzae via Introducing N-Glycosylation.
Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.
Cloning, large-scale production and characterization of fusion protein (P-TUFT-ALT-2) of Brugian abundant larval transcr
Synthesis, characterization and application of magnetoferritin nanoparticle by using human H chain ferritin expressed by
Improving control in microbial cell factories: from single cell to large-scale bioproduction
Production of <i>Rhizopus oryzae</i> lipase using optimized <i>Yarrowia lipolytica</i> expression system
The<i>Pichia pastoris</i>transmembrane protein GT1 is a glycerol transporter and relieves the repression of glycerol on
Yeast-based vaccines: New perspective in vaccine development and application
Glycosylation decreases aggregation and immunogenicity of adalimumab Fab secreted from Pichia pastoris.
The Impact of Methanol Concentration on Recombinant Protein Glycosylation in Pichia pastoris SuperMan5.
Isolation of a laccase-coding gene from the lignin-degrading fungus<i>Phlebia brevispora</i>BAFC 633 and heterologous ex
Expression, Docking, and Molecular Dynamics of Endo-<i>β</i>-1,4-xylanase I Gene of<i>Trichoderma virens</i>in<i>Pichia
Pichia pastoris: SEL RAGI UNTUK PRODUKSI PROTEIN REKOMBINAN
Xylanase gene mutation by error-prone pcr and expression in Pichia pastoris
The Effect Of Temperature On Recombinant Human Granulocyte Colony Stimulating Factor Production By Pichia pastoris Expre
Cloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudo
Cloning and Expression of an Active Aspartic Proteinase Gene From Aspergillus Oryzae DRDFS13 in Pichia Pastoris
Metabolic Engineering of the Methylotrophic Yeast Pichia Pastoris (Komagataella phaffii) for the Production of Β-alanine
Improving phytase production in Pichia pastoris fermentations through de-repression and methanol induction optimisation
Study of glycerol-methanol and sorbitol-methanol mixed feed strategies for recombinant hPDGF-BB production in Pichia pas
Effect of High-Speed Shaking on Oxygen Transfer in Shake Flasks.
Reinventing shake flask fermentation: The breathable flask.
Exploration of the Out‐of‐Phase Phenomenon in Shake Flasks by CFD Calculations of Volumetric Power Input, k<sub>L</sub>a
Development of a Jacketed Breathable Shake Flask With Process Monitoring, Control, and Bioreactor‐Like Performance
Physiological state as transferable operating criterion to improve recombinant protein production in <i>Pichia pastoris<
Oxygen transfer as a tool for fine-tuning recombinant protein production by Pichia pastoris under glyceraldehyde-3-phosp
Scale-up and fed-batch cultivation strategy for the enhanced co-production of microbial lipids and carotenoids using ren
Hydrodynamics and Mass Transfer Analysis in BioFlow® Bioreactor Systems
Avaliação da produção de xilitol em hidrolisado hemicelulósico de bagaço de cana de açúcar por linhagens de Cyberlindner
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Dextranase production by recombinant Pichia pastoris under operational volumetric mass transfer coefficient (kLa) and vo
Estrategia de control para la concentración de oxígeno disuelto en un biorreactor
Enhancing isoamyl acetate biosynthesis by Pichia fermentans
Microfluidic Microbioreactor to Reduce the Cost and Speed Up Optimisation of Protein Production
First-Principle Study of Gas Dispersion and Oxygen Transfer Efficiency in Non-Newtonian Fluids Using Coaxial Mixers
Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells
Produção de L-asparaginase de interesse farmacêutico a partir de leveduras isoladas do continente Antártico
Production and Purification of Soy Leghemoglobin from Pichia pastoris Cultivated in Different Expression Media
On-line methanol sensor system development for recombinant human serum albumin production by Pichia pastoris
Applied Microbiology 2016- Pichia process optimization by methanol/sorbitol co-feeding – Patrick Fickers - Universi
Bioprocess efficiency as a roadmap to establish Pichia pastoris as a reliable cell factory
Evaluating Pulses and Modified Fed-batch Feeding of Methanol to Increase Expression Level of Human Insulin Precursor in
HBsAg Production in Methanol Controlled <i>P. pastoris</i> GS115 Mut<sup>S</sup> Bioreactor Process
Physiology of aerobic yeast cultures under industrially relevant conditions
High-yield intracellular production of an exo-polygalacturonase enzyme via heterologous expression of Penicillium notatu
Pathogen-induced cell wall remodeling and production of Danger Associated Molecular Patterns (DAMPs)
Conformational Bias in 2'-Selenium-Modified Nucleosides and the Effect on Helical Structure and Extracellular Recom
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
Co‐Expression of Chaperones for Improvement of Recombinant <i>Geobacillus stearothermophilus</i> Lipase Production in <i
Biocatalytic synthesis of 2-O-α-D-glucopyranosyl-L-ascorbic acid using an extracellular expressed α-glucosidase from Ory
Process development for the continuous production of heterologous proteins by the industrial yeast, <i>Komagataella phaf
Physiological responses of <i>Saccharomyces cerevisiae</i> to industrially relevant conditions: Slow growth, low pH, and
Characterization of methanol utilization negative <i>Pichia pastoris</i> for secreted protein production: New cultivatio
Evolving trends in mAb production processes
Optimization of norovirus virus‐like particle production in <i>Pichia pastoris</i> using a real‐time near‐infrared biopr
Biotransformation of β‐hydroxypyruvate and glycolaldehyde to l ‐erythrulose by <i>Pichia pastoris</i> strain GS115 overe
Effect of Glycosylation on the Biocatalytic Properties of Hydroxynitrile Lyase from the Passion Fruit, Passiflora edulis
A novel strategy and kinetics analysis of half‐fractional high cell density fed‐batch cultivation of <i>Zygosaccharomyce
Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in
Development of a dual specific growth rate-based fed-batch process for production of recombinant human granulocyte colon
Bioprocess development for extracellular production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris.
Parallelized microscale fed-batch cultivation in online-monitored microtiter plates: implications of media composition a
De novo biosynthesis of 2'-fucosyllactose in engineered Pichia pastoris.
Comparative Study on Different Expression Hosts for Alkaline Phytase Engineered in Escherichia coli.
A Novel Methanol-Free Platform for Extracellular Expression of rhGM-CSF in Pichia pastoris.
β-glucosidase production by recombinant Pichia pastoris strain Y1433 under optimal feed profiles of fed-batch cultivatio
Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies
Improvement of catalytical properties of two invertases highly tolerant to sucrose after expression in Pichia pastoris.
Characterization of highly gelatinous patatin storage protein from Pichia pastoris.
In situ microscopy for online monitoring of cell concentration in Pichia pastoris cultivations.
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen.
Large-scale production of yak (Bos grunniens) chymosin A in Pichia pastoris.
Principal elementary mode analysis (PEMA)
Stability of Curcuma longa rhizome lectin: Role of N-linked glycosylation.
Fine‐tuning the <i>P. pastoris</i> iMT1026 genome‐scale metabolic model for improved prediction of growth on methanol or
Exploring Yeast's Energy Dynamics: The General Stress Response Lowers Maintenance Energy Requirement
Effect of Glycosylation of β-Glucuronidase on its Catalytic Properties in Ionic Liquids
High-density Cultivation in the Production of Microbial Products
Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Scale-up Production and Immunological Assessment of Recombinant SARS- CoV-2 Spike RBD in Pichia pastoris
Heterologous and efficient expression of a new alkaline pectin lyase in Pichia pastoris
Methanol Feeding Strategies for High-Yield Production of a Collagen-Based Protein in Komagataella phaffii
Heterologous Expression of Phanerochaete Chrysosporium Cellobiose Dehydrogenase in Trichoderma Reesei
Controlling specific growth rate for recombinant protein production by Pichia pastoris under oxidation stress in fed-bat
Characterization of recombinant Bacillus halodurans CM1 xylanase produced by Pichia pastoris KM71 and its potential appl
Enhanced human lysozyme production by Pichia pastoris via periodic dissolved oxygen concentration control
Extracellular expression and characterization of an α-glucosidase from Oryza sativa and its transglycosylation for synth
Metabolic heat response of Kluyveromyces marxianus during Carboxypeptidase Y Production; Effect of Agitation, Aeration a
Expression Systems for Synthetic Spider Silk Protein Production
Microfluidic Microbioreactor to Reduce the Cost and Speed Up Optimisation of Protein Production
Eukaryotic Expression and Purification Technology of Glucosidase in <i>Pichia Pastoris</i>
A comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii
Investigation into struvite precipitation: A commonly encountered problem during fermentations on chemically defined med
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
<i>Candida albicans</i> chitinase 3 with potential as a vaccine antigen: production, purification, and characterisation
Comparative thermal inactivation analysis of Aspergillus oryzae and Thiellavia terrestris cutinase: Role of glycosylatio
Functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution.
Advanced monitoring and control of pharmaceutical production processes with <i>Pichia pastoris</i> by using Raman spectr
Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastori
High-level production of recombinant HBcAg virus-like particles in a mathematically modelled P. pastoris GS115 Mut+ bior
Towards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologi
Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris
Nanobodies to multiple spike variants and inhalation of nanobody-containing aerosols neutralize SARS-CoV-2 in cell cultu
A high-yield xylanase platform for efficient xylooligosaccharides production from agricultural residues.
Genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by Aspergillus oryzae RI
Engineering Patatin for enhanced lipase activity and long-chain fatty acid specificity via rational design.
An impact of N-glycosylation on biochemical properties of a recombinant α-amylase from Bacillus licheniformis.
Elucidation of structure-function relationship of THCA and CBDA synthase from Cannabis sativaL.
Functional expression of recombinant goat chymosin in Pichia pastoris bioreactor cultures: A commercially viable alterna
Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice
Screening of a genome‐reduced <i>Corynebacterium glutamicum</i> strain library for improved heterologous cutinase secret
Production of codon-optimized Factor C fragment from Tachypleus gigas in the Pichia pastoris GS115 expression system for
Technical transfer of a rapid microbial platform for vaccine production
An integrative approach for high level production of glucuronoyl esterase in Pichia pastoris.
Development and characterization of fused human arginase I for cancer therapy
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
Holistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in <i>Pichia past
Production of Full-Length Antibody by Pichia pastoris.
Saccharomyces cerevisiae asparaginase II, a potential antileukemic drug: Purification and characterization of the enzyme
Extracellular production of the recombinant bacterial transglutaminase in Pichia pastoris
Structural and Biochemical Studies Enlighten the Unspecific Peroxygenase from <i>Hypoxylon</i> sp. EC38 as an Efficient
Improving the catalytic activity of a detergent‐compatible serine protease by rational design
Production Optimization of an Active β -Galactosidase of<i> Bifidobacterium animalis</i> in Heterologous Expression Syst
[Cloning of Oryza sativa N-glycanase gene (OsPNGase A) and its expression in Pichia pastoris].
Expression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose Host
Optimization of human chaperone BiP production in yeast Pichia pastoris
Farklı pH ve sıcaklık değerlerinin Pichia pastoris'de rekombinant ekspanzin üretimi üzerine etkisi
Nitrogen source and pH interact and modulate lipase secretion in a non-clinical strain of Candida parapsilosis
Determination of Putative Vacuolar Proteases, PEP4 and PRB1 in a Novel Yeast Expression Host Meyerozyma guilliermondii S
Overcoming Obstacles in Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field.
Production of 1, 12- dodecanedioic acid by recombinant microbial cytochrome P450 in pichia pastoris and saccharomyces ce
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
Functionalized silk assembled from a recombinant spider silk fusion protein (Z-4RepCT) produced in the methylotrophic ye
Metabolic modeling to identify engineering targets for <i>Komagataella phaffii</i>: The effect of biomass composition on
Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose‐regulated <i>GTH
Biosensor‐assisted engineering of a high‐yield <i>Pichia pastoris</i> cell‐free protein synthesis platform
Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies
Heterologous expression, purification, and functional characterization of recombinant ovine angiotensinogen in the methy
Recombinant horseradish peroxidase variants for targeted cancer treatment
Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications.
Comparing the Catalytic and Structural Characteristics of a ‘Short’ Unspecific Peroxygenase (UPO) Expressed in <i>Pichia
Programmable Biosynthesis of Plant-Derived 4'-Deoxyflavone Glycosides by an Unconventional Yeast Consortium.
Production of Therapeutic Single-Chain Variable Fragments (ScFv) in Pichia pastoris.
A novel Aspergillus oryzae diglycosidase that hydrolyzes 6-O-α-L-rhamnosyl-β-D-glucoside from flavonoids.
Substrate affinity and catalytic efficiency are improved by decreasing glycosylation sites in Trichoderma reesei cellobi
Rational design for the stability improvement of Armillariella tabescens β-mannanase MAN47 based on N-glycosylation modi
Comparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia past
Disrupting putative N-glycosylation site N17 in lipase Lip11 of Yarrowia lipolytica yielded a catalytically efficient an
Biochemical characterization of Aspergillus oryzae recombinant α-l-rhamnosidase expressed in Pichia pastoris.
Whole-cell (+)-ambrein production in the yeast Pichia pastoris
High-yield fermentation and a novel heat-precipitation purification method for hydrophobin HGFI from Grifola frondosa in
Downstream process and evaluation of the concomitant impact of a recombinant glycosylated L-asparaginase on leukemic can
Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
What makes <i>Komagataella phaffii</i> non-conventional?
Unraveling the potential of non-conventional yeasts in biotechnology
A universal gene expression system for fungi
Metabolic engineering of thermophilic <i>Bacillus methanolicus</i> for riboflavin overproduction from methanol
Knockout of <i>RSN1</i> , <i>TVP18</i> or <i>CSC1</i> <i>‐2</i> causes perturbation of Golgi cisternae in <i>Pichia past
N-Glycosylation Improves the Pepsin Resistance of Histidine Acid Phosphatase Phytases by Enhancing Their Stability at Ac
Improved Production of<i>Aspergillus usamii</i>endo-<i>β</i>-1,4-Xylanase in<i>Pichia pastoris</i>via Combined Strategie
<i>Aspergillus nidulans</i>: A Potential Resource of the Production of the Native and Heterologous Enzymes for Industria
Evaluation of Protective Effect of Naringenin on Cadmium-Induced Kidney Injury in Rats
Development of Higher Molecular Weight of Recombinant Human Interferon Alpha-2a by Albumin Fusion Technology in Methilot
A seven-transmembrane methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenas
High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availab
Engineering Yeasts to Grow Solely on Methanol or Formic acid coupled with CO2 fixation
Recombinant human gelatin: expression and characterization
A Single-Vector CRISPR/Cas9 System for Genome Editing and Heterologous Enzyme Secretion in Saccharomyces cerevisiae: A C
Secretory Expression of Cyclohexanone Monooxygenase by Methylotrophic Yeast for Efficient Omeprazole Sulfide Bio-oxidati
Development of a Pichia pastoris-Based Expression System for High-Activity Anti-SARS-CoV-2 Neutralizing Antibodies
Engineering Saccharomyces Cerevisiae for the Production and Secretion of Affibody Molecules.
Expression and Purification of Snake Antivenom Peptide in Pichia Pastoris
Heterologous Expression and Characterization of a Thermostable Exo-β-D-Glucosaminidase from Aspergillus oryzae.
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
Expression and characterization of a cellobiohydrolase (CBH7B) from the thermophilic fungus Thielavia terrestris in Pich
Engineering a Highly Regioselective Fungal Peroxygenase for the Synthesis of Hydroxy Fatty Acids
Recombinant exochitinase of the thermophilic mould Myceliopthora thermophila BJA: Characteristics and utility in generat
Evaluation of Methylotrophic Yeast Ogataea thermomethanolica TBRC 656 as a Heterologous Host for Production of an Animal
Biochemical characterisation of glycosylated and deglycosylated forms of phytase from Cronobacter turicensis expressed i
Heterologous expression of Stlac2, a laccase isozyme of Setosphearia turcica, and the ability of decolorization of malac
A multi-plex protein expression system for production of complex enzyme formulations in <i>Trichoderma reesei</i>
Abolishing storage lipids induces protein misfolding and stress responses in <i>Yarrowia lipolytica</i>
A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family
Molecular Engineering Improves Antigen Quality and Enables Integrated Manufacturing of A Trivalent Subunit Vaccine Candi
Cloning and characterization of bgl6111 gene encoding β‐glucosidase from bagasse metagenome
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
Customizing amino acid metabolism of <i>Pichia pastoris</i> for recombinant protein production
Cloning and Overexpressing Membrane Proteins Using <i>Pichia pastoris (Komagataella phaffii)</i>
High-level production of patatin in Pichia pastoris and characterization of N-glycosylation modification in food process
Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, co
Molecular engineering of insulin for recombinant expression in yeast
Recombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimi
Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expr
Expression of novel acidic lipase from Micrococcus luteus in Pichia pastoris and its application in transesterification
Microfluidic Screening And Genomic Mutation Identification For Enhancing Cellulase Production in Pichia Pastoris
Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates
Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii
Site-directed mutagenesis and thermal stability analysis of phytase from Escherichia coli
Expression and purification of recombinant human granulocyte colony‐stimulating factor (rG‐CSF) from Pichia pastoris
The Investigation of Clone and Expression of Butyrylcholinesterase in Pichia pastoris for Parathion Hydrolysis
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
<i>Pichia pastoris</i>: A highly successful expression system for optimal synthesis of heterologous proteins
A multistrategy approach for improving the expression of <i>E. coli</i> phytase in <i>Pichia pastoris</i>
Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges.
Role of N-glycosylation of surfactant protein SP-BN in lipid and SP-B interacting properties. Implications in disease.
Insights on Heterologous Expression of Fungal Cellulases in &lt;i&gt;Pichia pastoris&lt;/i&gt;
Expression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose Host
Gene and process level modulation to overcome the bottlenecks of recombinant proteins expression in Pichia pastoris.
Optimization of expression host for Thermomyces Lanuginosus lipase
Plant aquaporin reconstituted proteoliposomes as nanosystem for resveratrol encapsulation
Safety of the proposed amendment of the specifications for steviol glycosides (E 960) as a food additive: Rebaudioside M
Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol.
Purification and Characterization of β-Mannanase Derived from Rhizopus microsporus var. rhizopodiformis Expressed in Kom
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
The growth of Pichia pastoris Mut+ on methanol-glycerol mixtures fits to interactive dual-limited kinetics: model develo
Optimization of dilution rate and mixed carbon feed for continuous production of recombinant plant sucrose:sucrose 1-fru
Hybrid Deep Modeling of a GS115 (Mut+) Pichia pastoris Culture with State–Space Reduction
Applied Microbiology 2016- Pichia process optimization by methanol/sorbitol co-feeding – Patrick Fickers - Universi
Bioprocess efficiency as a roadmap to establish Pichia pastoris as a reliable cell factory
Integrating data-driven strategies into model predictive control for enhanced production of human interferon α2b in glyc
Optimizing Yeast Surface-Displayed Unspecific Peroxygenase Production for Sustainable Biocatalysis
Development of simple kinetic models and parameter estimation for simulation of recombinant human serum albumin producti
Cellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit
An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Bioprospecting the Regional Diversity of Australian Wine Microbiota
Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farn
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Developing strategies for systems metabolic engineering of Pichia pastoris
Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolyti
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition
Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous h
Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Development of Saccharomyces cerevisiae as a recombinant antibody factory
Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in <i>Pichia Pasto
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Investigating the impact of MpAPr1, an aspartic protease from the yeast Metschnikowia pulcherrima, on wine properties
Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Methods for production of recombinant enzymes for collagen degradation
An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreak
Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially thera
Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia
RSM optimization of HSA/IL1Ra in <i>Pichia pastoris</i> overexpression strain and study of its <i>in vivo</i> activity i
Modular development enables rapid design of media for alternative hosts
The optimization of fermentation conditions for <i>Pichia pastoris</i> GS115 producing recombinant xylanase
Comparative study of <i>μ</i>‐stat methanol feeding control in fed‐batch fermentation of <i>Pichia pastoris</i> producin
Recombinant production of a hard‐to‐express membrane‐bound cytochrome P450 in different yeasts—Comparison of physiology
Rapid screening of cellular stress responses in recombinant <i>Pichia pastoris</i> strains using metabolite profiling
Evaluation of the sub-optimal induction strategies for heterologous proteins production by Pichia pastoris Mut+/MutS str
High-level secretion and characterization of cyclodextrin glycosyltransferase in recombinant Komagataella phaffii.
Optimization of the fermentation parameters for the production of Ganoderma lucidum immunomodulatory protein by Pichia p
High level extracellular production of recombinant human interferon alpha 2b in glycoengineered Pichia pastoris: culture
Expresión y producción de la lacasa POXA 1B recombinante de pleurotus ostreatus como alternativa para el tratamiento de
Realm of Thermoalkaline Lipases in Bioprocess Commodities
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda
Optimization of Fermentation Conditions for Recombinant Lipase Expression At Small Scale Using Response Surface Methodol
Modular development enables rapid design of media for alternative hosts
Identifying bottleneck reactions and developing a systemic fed-batch feeding strategy of Pichia pastoris through fine-tu
Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacte
Evaluating the Application Potential of a Recombinant Ganoderma Protein as Bioactive Ingredients in Cosmetics
Upgrading Non-Conventional Yeasts into Valuable Biofactories
A seven-transmembrane methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenas
Engineering the conserved and noncatalytic residues of a thermostable β-1,4-endoglucanase to improve specific activity a
Biochemical analysis of Komagataella phaffii oxidative folding proposes novel regulatory mechanisms of disulfide bond fo
Characterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophic yeast Pichia pastori
High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris
Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in proces
Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall break
Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia p
Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastor
Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide iso
Genetic and physiological basis for antibody production by Kluyveromyces marxianus
A Recombinant Human Anti-Platelet scFv Antibody Produced in Pichia pastoris for Atheroma Targeting
Rekombinante Produktion eines Kollagen-mimetischen Proteins in Komagataella phaffii (syn. Pichia pastoris)
Fusion Partner Facilitates Expression of Cell-Penetrating Peptide L2 in Pichia pastoris
Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds.
Pichia pastoris (Komagataella phaffii) as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countr
Cutinase Production in Komagataella phaffii (Pichia pastoris): Performance Differences Between Host Strains
Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a H
Expression of functional human sialyltransferases ST6GalNAc5 and ST6GalNAc6 in Pichia pastoris
Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale
Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol meta
An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibri
Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella ph
Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Re
Study of pH and Thermodynamic Parameters via Circular Dichroism Spectroscopy of a Recombinant Human Lactoferrin
Biological Activity of Optimized Codon Bovine Type III Interferon Expressed in Pichia pastoris.
Cell Surface Display of Red-Grouper Nervous Necrosis Virus Capsid Protein on &lt;i&gt;Pichia pastoris&lt;/i&
Two adjacent C-terminal mutations enable expression of aryl-alcohol oxidase from Pleurotus eryngii in Pichia pastoris
Hyperosmolarity adversely impacts recombinant protein synthesis by Yarrowia lipolytica—molecular background revealed by
Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzym
Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indica
Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational
Purification and characterization of a novel phloretin-2'-O-glycosyltransferase favoring phloridzin biosynthesis.
Modular biosynthesis of plant hemicellulose and its impact on yeast cells
Automated Feeding and Fermentation Phase Transitions for the Production of Unspecific Peroxygenase by <i>Pichia pastoris
Characterization of Recombinant Human Lactoferrin Expressed in <i>Komagataella Phaffii</i>
Comparative transcriptome and metabolome analyses reveal the methanol dissimilation pathway of Pichia pastoris
Engineered Pichia pastoris production of fusaruside, a selective immunomodulator
Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombol
Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol
Novel homologous lactate transporter improves l-lactic acid production from glycerol in recombinant strains of Pichia pa
Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae
Elimination of N-glycosylation by site mutation further prolongs the half-life of IFN-α/Fc fusion proteins expressed in
Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryot
Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris
Modular biosynthesis of plant hemicellulose and its impact on yeast cells
Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermoph
Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine
An improved expression and purification protocol enables the structural characterization of Mnt1, an antifungal target f
Heterologous expression of the antimyotoxic protein DM64 in Pichia pastoris.
Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with R
Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus e
Biological Activity of Optimized Codon Bovine Type III Interferon Expressed in Pichia Pastoris
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD
Identification and Characterization of a Novel Hyperthermostable Bifunctional Cellobiohydrolase- Xylanase Enzyme for Syn
Combining Metabolic Engineering and Multiplexed Screening Methods for 3-Hydroxypropionic Acid Production in Pichia pasto
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter
Characterization of Full-Length and Truncated Recombinant κ-Carrageenase Expressed in Pichia pastoris
A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris
Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris.
Gene Expression, Biochemical Characterization of a sn-1, 3 Extracellular Lipase From Aspergillus niger GZUF36 and Its Mo
Design and High Expression of Non-glycosylated Lysostaphins in Pichia pastoris and Their Pharmacodynamic Study.
Data_Sheet_1_Heterologous Expression and Characterization of a High-Efficiency Chitosanase From Bacillus mojavensis SY1
Yeast Secretes High Amounts of Human Calreticulin without Cellular Stress
Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pasto
Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endo
Improved Production of Recombinant Myrosinase in Pichia pastoris
Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unve
A Lipase Gene of Thermomyces lanuginosus: Sequence Analysis and High-Efficiency Expression in Pichia pastoris.
Comparison of Activity and Safety of DSPAα1 and Its N-Glycosylation Mutants.
Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin
Novel multiphase loop reactor with improved aeration prevents excessive foaming in Rhamnolipid production by Pseudomonas
A CONTROL OF OXYGEN SUPPLY IN BIOCONVERSION OF SUGARCANE TRASH INTO XYLITOL BY Meyerozyma guilliermondii InaCCY65
Bacillus subtilis NCIM2063 batch cultivation: The influence of the substrate concentration and oxygen transfer rate on t
Development and Optimization of a Laboratory-Scale Bubble Column Bioreactor for Bioethanol Fermentation: A Computational
Otimização da produção de lipídeos por <i>Rhodotorula glutinis</i> e aumento de escala em biorreatores de agitação pneum
L-asparaginase de Erwinia chrysanthemi expressa em sistema Pichia pastoris: otimização das condições de cultivo em biorr
Production of L-asparaginase of pharmaceutical nterest from yeasts isolated from the Antarctic continent
Modelado de la transferencia de masa a diferentes escalas en la producción de soforolípidos a nivel laboratorio
Development and optimization of a fermentation process for the production of 2,3-butanediol
Scale up of Enhanced Green Fluorescent Protein (EGFP) production from recombinant Escherichia coli in conventional biore
Understanding changes in microbial metabolism of Clostridium termitidis under different growth conditions using continuo
Optimization of growth and induction conditions for the production of recombinant whole cell cyclohexanone monooxygenase
Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production
Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with mo
Microbubble assisted polyhydroxybutyrate production in Escherichia coli
Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures
Existence of a scaling relation in continuous cultures of Scheffersomyces stipitis: the steady states are completely det
‘Small volume—big problem’: culturing Yarrowia lipolytica in high-throughput micro-formats
Improved production of Daptomycin in an airlift bioreactor by morphologically modified and immobilized cells of Streptom
Volumetric Oxygen Transfer Coefficient Effect on Biomass, Bioactive Compounds Production, and Kinetic Behavior of G. luc
Designing of Fermenter and its utilization in food industries
ВЛИЯНИЕ МЕХАНИЗМОВ ДИВЭ НА СКОРОСТЬ МАССОПЕРЕНОСА И РОСТ МИКРООРГАНИЗМОВ
Improvement of Saccharomyces propagation performance through oxygen-enriched air and aeration parameter variation
kLa based scale-up cultivation of the extremophilic archaeon Sulfolobus acidocaldarius: from benchtop to pilot scale
Tools for reactor evaluation in bioprocesses
Potentials of utilizing renewable resources of agriculture residues for the biosynthesis of bacterial cellulose nanofibe
Production and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris
Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammali
Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air
Metabolic and Process Engineering of Pichia Pastoris for the Production of Value-added Products
Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia
Microbioreactors and Perfusion Bioreactors for Microbial and Mammalian Cell Culture
A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris
Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii
Extracellular expression, purification and bioreactor production of tambaqui (Colossoma macropomum) growth hormone in th
Leveraging Historical Process Data for Recombinant <i>P. pastoris</i> Fermentation Hybrid Deep Modeling and Model Predic
Continuous Process Verification 4.0 application in upstream: adaptiveness implementation managed by AI in the hypoxic bi
Glycosylation Contributes to Thermostability and Proteolytic Resistance of rFIP-nha (Nectria haematococca)
Improved Production of Recombinant Carboxylesterase FumDM by Co-Expressing Molecular Chaperones in Pichia pastoris
Cloning, characterization and directed evolution of a xylosidase from Aspergillus niger
Structure and function studies of GH45 glycoside hydrolases
Production Processes for Monoclonal Antibodies
High cell density fermentation strategies for the production of bovine lactoferrin in Pichia pastoris
Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional chara
Methanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the h
Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B
P <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia
Optimality of extracellular enzyme production and activity in dynamic flux balance modeling
<i>Pichia pastoris</i> growth - coupled heme biosynthesis analysis using metabolic modelling
Characterising the metabolic rewiring of extremely slow growing <i>Komagataella phaffii</i>
Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris
Production of human pro-relaxin H2 in the yeast Pichia pastoris
Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia p
Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella
High-level production of Aspergillus niger prolyl endopeptidase from agricultural residue and its application in beer br
Unlocking Nature’s Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promot
Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application i
Crystal Structure of Alcohol Oxidase from Pichia pastoris
Cofactor engineering improved CALB production in Pichia pastoris through heterologous expression of NADH oxidase and ade
Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol
Bioprocess optimization of interferon β-1-a in Pichia pastoris and its improved inhibitory effect against hepatocellular
Signal Peptide Selection for the Efficient Periplasmic and Secretive Expression of Recombinant Brazzein in Escherichia C
Heterologous Expression of Histidine Acid Phytase From Pantoea sp. 3.5.1 in Methylotrophic Yeast Pichia Pastoris
Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized p
Secretory expression of the recombinant FGF-2 protein in Pichia pastoris carrying multiple copies of target gene
High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia past
Developing methylotrophic microbial platforms for a methanol-based bioindustry
Structural analysis and functional evaluation of the disordered ß–hexosyltransferase region from Hamamotoa (Sporobolomyc
Biochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mann
The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments
Secreted Expression of Thymosin β4 from Pinctada fucata in Pichia pastoris and Its Biological Activity
Optimization of Bioethanol In Silico Production Process in a Fed-Batch Bioreactor Using Non-Linear Model Predictive Cont
Modifications of Constitutive Promoter to Large-Scale Synthesize Porcine Myoglobin in Komagataella phaffii
Use of Pressurized and Airlift Bioreactors for Citric Acid Production by Yarrowia lipolytica from Crude Glycerol
Model Predictive Control—A Stand Out among Competitors for Fed-Batch Fermentation Improvement
High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity.
Overexpression and Biochemical Characterization of an Endo-α-1,4-polygalacturonase from Aspergillus nidulans in Pichia p
A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production
Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in Pichia pastoris for Biomedical Applications
Comparison of Different Signal Peptides for the Efficient Secretion of the Sweet-Tasting Plant Protein Brazzein in Pichi
Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production
Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms
Enhancing recombinant T1 lipase production in Pichia guilliermondii
Development of next-generation biopesticides as a control method for the small hive beetle Aethina tumida murray (coleop
Accelerating cell culture media development using Bayesian optimization-based iterative experimental design
Structural and Functional Comparison of SARS-CoV-2-Spike Receptor Binding Domain Produced in <i>Pichia pastoris</i> and
A vaccine consisting of Schistosoma mansoni cathepsin B formulated in Montanide ISA 720 VG induces high level protection
Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermenta
Protein behavior in crowded environments
Yeast-derived low-purity FGF2 supports bovine ESC and MSC aggregates in suspension
Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis
Intensification of Recombinant Laccase Production from Sago Bioethanol Liquid Waste and Evaluation of The Enzyme for Syn
FeedER: a feedback-regulated enzyme-based slow-release system for fed-batch cultivation in microtiter plates
Enzymatic activity of a recombinant β-1,4-endoglucanase from the Cotton Boll Weevil (Anthonomus grandis) aiming second g
Dissolved-oxygen feedback control fermentation for enhancing β-carotene in engineered Yarrowia lipolytica
High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization,
Improving the catalytic performance of <i>Pichia pastoris</i> whole-cell biocatalysts by fermentation process
Steric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>K
Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant
Engineering a carbohydrate-binding module to increase the expression level of glucoamylase in Pichia pastoris
Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella
Expression and characterization of cold-adapted xylanase Xyl-L in Pichia pastoris for xylooligosaccharide (XOS) preparat
Improved spectrophotometric assay for lytic polysaccharide monooxygenase
Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separati
Obtaining of Recombinant Camel Chymosin and Testing Its Milk-Clotting Activity on Cow’s, Goat’s, Ewes’, Camel’s and Mare
Efficient Secretory Expression for Mammalian Hemoglobins in Pichia pastoris
Coriolopsis trogii MUT3379: A Novel Cell Factory for High-Yield Laccase Production
Lipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications
High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris
Optimization Workflow of Fumonisin Esterase Production for Biocatalytic Degradation of Fumonisin B1
Expression and Characterization of an Alginate Lyase and Its Thermostable Mutant in Pichia pastoris
Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein E
Characterization of a novel aspartic protease from Trichoderma asperellum for the preparation of duck blood peptides
Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy
Engineering a recombination neutral protease I from <i>Aspergillus oryzae</i> to improve enzyme activity at acidic pH
Time-resolved secretome analysis of three <i>Colletotrichum</i> species identifies copper radical alcohol oxidases for t
Clonagem, expressão heteróloga e caracterização funcional de peptidase de Fusarium oxysporum em Pichia pastoris
Transcriptoma e expressão recombinante de peptidases do fungo Phanerochaete chrysosporium em Pichia pastoris
Expressão e caracterização das proteínas VP1 e VP2 de parvovírus humano B19 em <i>Pichia pastoris</i>.
Expression, purification and biological characterization of the extracellular domain of CD40 from Pichia pastoris
A high-throughput expression screening platform to optimize the production of antimicrobial peptides
Saccharomyces cerevisiae as host for the recombinant production of polyketides and nonribosomal peptides
Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection
Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
Synthetic methylotrophic yeasts for the sustainable fuel and chemical production
Expression of a highly active β-glucosidase from Aspergillus niger AS3.4523 in Escherichia coli and its application in g
YEASTS BIOTECHNOLOGIES APPLICATION AND THEIR INVOLVING IN AFRICAN TRADITIONAL FERMENTED FOODS AND BEVERAGES.
Multi-Level Optimization and Strategies in Microbial Biotransformation of Nature Products
Engineering a New IFN-ApoA-I Fusion Protein with Low Toxicity and Prolonged Action
Optimization of a Recombinant Lectin Production in Pichia pastoris Using Crude Glycerol in a Fed-Batch System
Development and Optimization of a Novel Soft Sensor Modeling Method for Fermentation Process of Pichia pastoris
A Delta–Omicron Bivalent Subunit Vaccine Elicited Antibody Responses in Mice against Both Ancestral and Variant Strains
The Use of α-MF Signal Peptide Without Spacer for Producing Insulin Aspart Precursor in <i>Pichia pastoris</i> KM71
Engineering of small laccase (SLAC) from Streptomyces coelicolor for application in biocatalysis and surface functionali
Cloning and characterisation of three novel lipases from Thermomyces lanuginosus
Targeted Deletion of Los1 Homologue Affects the Production of a Recombinant Model Protein in Pichia pastoris
Direct gamete interactions and EGG CELL1-mediated sperm cell activation in Arabidopsis thaliana
Talaromyces marneffei Cu, Zn Superoxide Dismutase Recombinant Protein Expression in Pichia pastoris, Enzymatic Activity
Expression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenet
Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints
Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris
Altered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergil
Multi-omics integrative analysis with genome-scale metabolic model simulation reveals global cellular adaptation of Aspe
Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris
A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
Pathway elucidation of bioactive rhamnosylated ginsenosides in Panax ginseng and their de novo high-level production by
Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris
Biotechnological production of the mussel byssus derived collagen preColD
Biochemical, structural insights of newly isolated AA16 family of Lytic Polysaccharide Monooxygenase (LPMO) from <i>Aspe
A modular two yeast species secretion system for the production and preparative application of fungal peroxygenases
Process Development and Scale-up Optimization of the SARS-CoV-2 Receptor Binding Domain-Based Vaccine Candidate, RBD219-
Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamster
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered <i>Komagataella phaffii</i
A membrane integral methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenases
Identification, characterisation and recombinant expression of flavonoid 3’,5’-hydroxylases and cytochrome P450 reductas
Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in
The Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </
Display of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produc
The adaptive landscape of wildtype and glycosylation-deficient populations of the industrial yeast Pichia pastoris
Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipo
Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and th
Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physio
Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins
Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant g
Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris
Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pa
Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitn
CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Whole-genome metabolic model of Trichoderma reesei built by comparative reconstruction
Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency
Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia past
Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macroph
High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia past
[Fundamental Research into Enhancing Fab Functionality by Protein Engineering].
Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca.
Modulation of heterologous protein secretion in the thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 6
Metabolism of Non-growing Cells and Their Application in Biotransformation
Analysis of a thermostable lipase from CTG-clade yeast: Molecular expression, characterization and structure prediction
Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris
Optimasi Waktu Induksi dalam Mengekspresikan Gen Proinsulin secara Intraseluler Menggunakan Inang Pichia pastoris
Nitrogen recovery from low-value biogenic feedstocks via steam gasification to methylotrophic yeast biomass
Vaccine Containing the Three Allelic Variants of the Plasmodium vivax Circumsporozoite Antigen Induces Protection in Mic
Murine Factor H Co-Produced in Yeast With Protein Disulfide Isomerase Ameliorated C3 Dysregulation in Factor H-Deficient
Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications.
Current advances of Pichia pastoris as cell factories for production of recombinant proteins
Enhancement of HSA-pFSHβ production by disrupting YPS1 and supplementing N-acetyl-L-cysteine in Pichia pastoris
High expression of antimicrobial peptides cathelicidin-BF in Pichia pastoris and verification of its activity
From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase
Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P4
Different N-Glycosylation Sites Reduce the Activity of Recombinant DSPAα2.
Efficient Secretory Expression of Leghemoglobin in Saccharomyces cerevisiae
Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview
Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Enhancing the Heterologous Expression of a Thermophilic Endoglucanase and Its Cost-Effective Production in Pichia pastor
Brassica oleracea L. var. italica Aquaporin Reconstituted Proteoliposomes as Nanosystems for Resveratrol Encapsulation
Sustainability Assessment in Recombinant Human Insulin Production—Evaluating the Environmental Impacts of Microbial Grow
Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories
Heterologous Hyaluronic Acid Production in Kluyveromyces lactis
Enhancing recombinant T1 lipase production in Pichia guilliermondii
Recombinant synthesis of pulmonary surfactant proteins SP-B and SP-C
Development of next-generation biopesticides as a control method for the small hive beetle Aethina tumida murray (coleop
Choline-Based Ionic Liquids as Media for the Growth of Saccharomyces cerevisiae
The Effect of Recombinant Tags on Citrus paradisi Flavonol-Specific 3-O Glucosyltransferase Activity
Foam control in biotechnological processes—challenges and opportunities
A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candi
The SARS-CoV-2 receptor-binding domain expressed in Pichia pastoris as a candidate vaccine antigen
Expressing Cloned Genes for Protein Production, Purification, and Analysis
Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina
Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed
Production of l-alanyl-l-glutamine by immobilized Pichia pastoris GS115 expressing α-amino acid ester acyltransferase
Production of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did n
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candi
Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris
Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa
Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-
Production of protein‐based polymers in Pichia pastoris
Constitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase
Broadening the Biocatalytic Toolbox—Screening and Expression of New Unspecific Peroxygenases
Tuning Fatty Acid Profile and Yield in Pichia pastoris
Depletion of Boric Acid and Cobalt from Cultivation Media: Impact on Recombinant Protein Production with Komagataella ph
Specific Phenylpropanoid Oligomerization in a Neutral Environment by the Recombinant Alkaline Laccase from Paramyrotheci
Mutation of cysteine residues increases heterologous expression of peach expansin in the methylotrophic yeast <i>Pichia
Cloning and expression of a GH11 xylanase from Bacillus pumilus SSP-34 in Pichia pastoris GS115: Purification and charac
High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances a
Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris
Designing strong inducible synthetic promoters in yeasts
Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on tra
Genome scale modeling of the protein secretory pathway reveals novel targets for improved recombinant protein production
Heterologous expression and biochemical characterization of a GHF9 endoglucanase from the termite Reticulitermes speratu
Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in
Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant protein
Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a
Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacill
Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii
Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastor
Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism
High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availab
‘Mother(Nature) knows best’ – hijacking nature-designed transcriptional programs for enhancing stress resistance and pro
Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates
High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate p
Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris
Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolis
Transcription regulation strategies in methylotrophs: progress and challenges
Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastoris
A quantitative indicator diagram for lytic polysaccharide monooxygenases reveals the role of aromatic surface residues i
The Achilles Heel of Protein Biochemistry: Insolubility of Recombinant Proteins—A Case Study About Producing a Rice Enzy
A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris
Molecular Modification of Kex2 P1’ Site Enhances Expression and Druggability of Fungal Defensin
An Interplay between Transcription Factors and Recombinant Protein Synthesis in Yarrowia lipolytica at Transcriptional a
The Achilles Heel of Protein Biochemistry: Insolubility of Recombinant Proteins—A Case Study About Producing a Rice Enzy
Metabolic Engineering of Pichia pastoris for the Production of Triacetic Acid Lactone
Recombinant Thaumatin-Like Protein (rTLP) and Chitinase (rCHI) from Vitis vinifera as Models for Wine Haze Formation
Constitutive expression and anticancer potency of a novel immunotoxin onconase-DV3
Yeast as a Versatile Tool in Biotechnology
Growth-coupled selection of synthetic modules to accelerate cell factory development
High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich
Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis
Process development for an effective COVID-19 vaccine candidate harboring recombinant SARS-CoV-2 delta plus receptor bin
Identification of a hot-spot to enhance<i>Candida rugosa</i>lipase thermostability by rational design methods
Improving the heterologous production of fungal peroxygenases through an episomal <i>Pichia pastoris</i> promoter and si
The trade-off of availability and growth inhibition through copper for the production of copper-dependent enzymes by Pic
Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein
Secretion of a low and high molecular weight β-glycosidase by Yarrowia lipolytica
Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulat
Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half
From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pa
Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in
Second generation Pichia pastoris strain and bioprocess designs
The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella p
Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pic
Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene
Improving the Secretory Expression of an α-Galactosidase from Aspergillus niger in Pichia pastoris
Cloning, expression, and characterization of a recombinant xylanase from Bacillus sonorensis T6
Nutrient biomass production from agro-industrial residues using Yarrowia lipolytica: screening and optimization of growi
Insight to Improve α-L-Arabinofuranosidase Productivity in Pichia pastoris and Its Application on Corn Stover Degradatio
Overexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for C
Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production
High-Level Expression and Engineering of Candida antarctica Lipase B in a Non-Methanol-Induced Pichia pastoris System
Heterologous Production, Purification and Characterization of Two Cold-Active β-d-Galactosidases with Transglycosylation
Comparison of Yeasts as Hosts for Recombinant Protein Production
Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor pr
Uncoupling protein production from growth: different strategies for intracellular and secreted proteins in yeast
Candida boidinii isolates from olive curation water: a promising platform for methanol-based biomanufacturing
Research on Soft-Sensing Method Based on Adam-FCNN Inversion in Pichia pastoris Fermentation
Statistical Improvement of Batch Culture with Immobilized <i>Pichia pastoris</i> Cells for rPOXA 1B Laccase Production
Improved β-galactosidase Secretion in Fresh Water Fungi through Cultural Optimization
Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-stat
Modular development enables rapid design of media for alternative hosts
A general deep hybrid model for bioreactor systems: combining first Principles equations with deep neural networks
Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otim
Clonagem e expressão de fragmentos de anticorpo de cadeia única (scFv) anti-LDL eletronegativa em <i>Pichia pastoris</i>
Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris
Genome wide analysis of protein production load in Trichoderma reesei
A Simplified and Efficient Process for Insulin Production in Pichia pastoris
New lipases by mining of Pleurotus ostreatus genome
Optimization of an Activity Assay of Coprinus Cinereus Peroxidase
Improvement of the Catalytic Ability of a Thermostable and Acidophilic β-Mannanase Using a Consensus Sequence Design Str
From Spatial-Temporal Multiscale Modeling to Application: Bridging the Valley of Death in Industrial Biotechnology
Production of a Human Histamine Receptor for NMR Spectroscopy in Aqueous Solutions
Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase f
Truncated Prosequence of Rhizopus oryzae Lipase: Key Factor for Production Improvement and Biocatalyst Stability
Bram; Schotte, Peter
Peter.schotte@sanofi.com
Optimizing expression of Nanobody <sup>®</sup> molecules in Pichia pastoris through co-expression of auxiliary proteins
Viewxszhou@ecust.edu.cn
Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shi
mervi.toivari@vtt.fi
Evaluation of synthetic formaldehyde and methanol assimilation pathways in Yarrowia lipolytica.
shun.satou@aist.go.jp
Preliminary Evaluation of Glyceric Acid-producing Ability of Acidomonas methanolica NBRC104435 from Glycerol Containing
diethard.mattanovich@boku.ac.at
Efficient production of itaconic acid from the single carbon substrate methanol with engineered <i>Komagataella phaffii<
simona.capone@tuwien.ac.at
Different approaches to make Pichia pastoris a more versatile expression platform - process, strain and product engineer
brigitte.gasser@boku.ac.at
Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in yeast
a.glieder@tugraz.at
Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9
myrsini.sakarika@ugent.be
The nutritional composition and cell size of microbial biomass for food applications are defined by the growth condition
e.keshavarz-moore@ucl.ac.uk
Enhancing Sustainability and Productivity in <em>Komagataella phaffii</em> Fermentation: A Techno-Economic Comparison of
dmitry.gradov@lut.fi
Experimentally validated numerical modelling of reacting multiphase flows in stirred tank reactors
cv.bustos@student.Uliege.be
Sorbitol uptake and oxygen transfer shape <i>AOX1</i> promoter induction under methanol-free conditions in <i>Komagatael
yuaiqun@tust.edu.cn
Enhanced production of amyrin in Yarrowia lipolytica using a combinatorial protein and metabolic engineering approach
nsoemenguenestor@yahoo.fr
<sup>Hydrodynamic Study and Influence of Physicochemical Parameters on Gas Retention in a Submerged Membrane Bioreactor<
m.frolov@knc.ru
Novel Silicone–Polyol Antifoam Emulsions: Impact on Foam Control and Physiology of Diverse Microbial Cultures
bsen@tju.edu.cn
Low dissolved oxygen supply functions as a global regulator of the growth and metabolism of Aurantiochytrium sp. PKU#Mn1
cedric.schirmer@zhaw.ch
Recommendation for biological evaluation of bioreactor performance for microbial processes
Mounia.akassou@usherbrooke.ca
Production d’une activité amylolytique thermostable par Thermus thermophilus HB8 suivie du clonage et de l’expression d’
fdamron@hsc.wvu.edu
Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS
yingli528@vip.sina.com
Efficient heterologous expression of an alkaline lipase and its application in hydrolytic production of free astaxanthin
thalyta.pacheco@embrapa.br
Targeted Redesign and Optimization of Culture Media for Ethylene Glycol Biosynthesis in Komagataella phaffii
Mounia.akassou@usherbrooke.ca
Production d’une activité amylolytique thermostable par Thermus thermophilus HB8 suivie du clonage et de l’expression d’
d.wetzel@artes-biotechnology.com
Display of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produc
ping.ho@connect.polyu.edu.hk
Efficient production of secretory Streptomyces clavuligerus β-lactamase inhibitory protein (BLIP) in Pichia pastoris
shinta.kusumawardani@biofarma.co.id
Defining a Simplified Process in Yeast for Production of Enveloped VLP Dengue Vaccine
paola.branduardi@unimib.it
Optimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti
lars.blank@rwth-aachen.de
Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha
Pau.Ferrer@uab.cat
Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol